Molecular profiling of 20 different accessions of Canna using RAPD and ISSR primers
Tanmayee Mishra, Arvind Kumar Goyal and Arnab Sen
Canna, the solitary genus of the family Cannaceae, represents a group of ornamental plants. In the present study an attempt was made to access the genetic relationship among the 20 species and cultivars of Canna using RAPD and ISSR techniques. A total of 159 major scorable bands ranging from 220 to 1757 bp were generated from 18 RAPD primers showing 89.93% polymorphism. The lowest similarity was observed between Canna edulis and Canna x generalis Cv. “Trinacria Variegata” (52.9%), while the highest value was recorded between Canna x generalis Cv. “Dwarf Red” and Canna x generalis Cv. “Dwarf Orange” and between Canna x generalis Cv. “Dwarf Orange” and Canna x generalis Cv. “Froken” (94.1%). Ten ISSR primers resulted in 93 scorable bands showing 88.17% polymorphism among them. The band size ranged between 246 bp to 2017 bp. The highest correlation was found between Canna edulis and its green cultivar (96.4%), where as lowest was found between Canna indica and Canna x generalis Cv. “Orange Web” (55.9%). The dendrogram based on the combined data sets of both RAPD and ISSR showed considerable similarity with that obtained from individual RAPD and ISSR analysis, except for the position of C. indica Cv. Purpurea.
KEYWORDS: Canna; Molecular diversity, PEG, RAPD, ISSR.